ANALYTICAL TECHNIQUES Thepresent study UV-Vis and HPLC profile of the Gracilaria species was evolved.Thus the present studies exhibited novel markers in standardization as usefulanalytical tools. In earlier reported thespectra for flavonoids typically lie in the range of 190-800 nm (Neha andJyoti, 2013). Zavoi et al.

, 2011reavealed that the peak value of 280 nm and 330 nm indicates the presence ofphenolic compound and its derivatives in the plant. The present result ofUV-VIS spectroscopic analysis and HPLC confirms the presence of phenoliccompounds of flavonoids in the chloroform and methanolic extract of Gracilaria species which indicates themedicinal properties of these seaweeds. Phenolic compound flavonoids used asAntioxidant Activity, Hepatoprotective, Anti-Inflammatory, Anticancer andAntiviral activity of this seaweed extract also observe by earlier SarikaKhandare et al.

, 2012. A number of studiesexplained the separation of phenolics and steroids using TLC (Hajimehdipoor et al., 2009).  In the present study also developed the TLCprofile for Gracilaria specieswhich demonstrated and obtain different RF values. Thin LayerChromatography (TLC) technique can be utilized for separation, isolation,identification and quantification of components in a mixture.Inspectral analysis, the data compared with the reported spectral values of theIR, NMR and GC-MS spectra.

The strong absorption bands at 3334.92 and 3423.65cm-1 OH and NH stretching vibrations in all Gracilaria species. The FTIR spectrum profile confirm several peaksare compounds of hydroxyl group (O-H stretch), NH Stretch, alkyl (C-H stretch),carbonyl ester (C=O stretch), alcohol, ether, acid (C-O) and amino group (NOstretch). The results of the present study confirms the presence of phenol,alkane, alkene, carboxylic acid, aromatic compound, nitro compound, alcohol,benzene and bromo alkanes compounds in Gracilaria species.  A similar finding wasreported earlier which showed the functional groups from bioactive compoundsextracted from S. wightii and U. lactuca (Meenakshi et al.

, 2012)GCMSChemical characterization and compositional analysis oftraditional medicines provide the necessary scientific basis for the discoveryand development of new drugs of natural origin (Shrestha and Dhillion, 2003) Inpreviously reported for various biological activities such as antimicrobial,anticancer, antiinflammatory and diuretic activity (Praveen et al., 2010).The phytol(2-Hexadecen-1-ol, 3, 7, 11, 15-tetramethyl-) is also an anticancer agent(colon and gastric cancer) and used in the preparation of vitamin E and K. Ithas potent antitumor activity against induced Raji cells at concentration of15µg/ml and 30µg/ml in the medium (Furumoto et al, 2002). Moreover, Eicosaneand 2-Hexadecen-1-ol, 3, 7, 11, 15- tetramethyl- are reported to be the mainconstituents in the Aloe vera extract; responsible for high antimicrobial activityagainst clinical pathogens (Arunkumar and Muthuselvam, 2009).

Octadene is alsosaid to possess various activities such as anticancer, antioxidant andantimicrobial activities (Vinay et al., 2011; Lee et al., 2007). The structureand kinetics studies of n-Hexedeconic acid (palmitic acid) revealed that it isan inhibitor of phospholipase, hence and anti-inflammatory compound (Aparna etal., 2012).Oleic acid (14.

58%)and n-hexadecanoicacid (24.73%) were the major compounds detected, along with the minor compoundsof octadecanoic acid, hexadecanoic acid, Z-11-, Cholestane 3,6-dione,(5, 17,20S)-, 1,2-Benzenedicarboxylic acid, bis (2- methylpropyl) ester, heneicosane,nonacosane and hexacosane. Results of our study matched with a compositionstudy of Acanthaphora spicifera from Pakistan in respect to the major compoundsof fatty acids detected, octadecadienoic acid (36.05%) and hexadecanoic acid(8.30%) analyzed by Laila, 2003.Praveen Kumarm et al., (2010) reported the activities of 9.12.15 octadecatrienoicacid, ethyl ester (z,z,z), ethyl palmitate and N-hexadecanoic acid asAnti-inflammatory, hypocholesterolemic cancer preventive, hepatoprotective,Antioxidant and hypocholesterolemic.