of ?-catenin. Theseresults propose that deletions occurring in FAT1 gene contributes to cancerousdispersed morphology. settlementNishikawa et al. analyzed the expression levels of mRNAs of FAT1, whichexist in human OSCC cell lines with RT-PCR. The levels showed diverse resultsfor all cultured cell lines except SAS and SCC66. Additionally, immunofluorescencewas conducted with the same aim. However, alike result got yield. Results gotfrom double immunofluorescence done with 3 FAT1-positive and 1 FAT1-negativecell lines showed that ?-catenin is localized in the cell membrane ofFAT1-expressing OSCCs, however in the FAT1-negative cell since the gene isabsent, ?-catenin’s got present only in cytoplasm and nuclei due to cell tocell adhesion being lack, hence have a undifferentiated shape of cells.
In thelight of points mentioned before; the gene FAT1 can be considered to be astumor suppressor. Additionally, the gene can regulate cell proliferation in anindirect way. Expression of gene is inversely related to proliferation in vascularmuscle cells When the expression of gene is restricted, proliferation invascular muscle cells gets increased; when the expression is promoted, the cellproliferation got decreased. Thus, it was predicted that suppression of thegene would also raise the proliferation of oral squamous cell carcinomas (OSCCs). However, in this study when the mRNAs ofFAT1 genes were silenced, circumscribed effects were observed on theproliferation of OSCC cell lines. Therefore, it can be concluded that FAT cadherinhas a more minor role for cell proliferation of OSCC when it is compared withother tumor suppressor genes.
Occurrence of deletion in the product of the geneFAT1 might have a major role in the metamorphosis of various characteristics ofcarcinoma cells including; decrement of cell to cell adhesion, morphology andcell polarity. One of the characteristic roles of gene FAT1 products is tomaintain morphology and polarity by collaborating with the stress fibers?-catenin and Vasp/Ena which binds with the cytoplasmic domain of the gene.Furthermore, the gene contains a nuclear localization signal sequence and bytranslocating its cytoplasmic domain into the nucleus, cell polarity andmigration cell polarity and migration can occur as result. This study alsorevealed that, when the gene FAT1 was silenced a significant reduction of cellto cell adhesion occurred alongside alteration in cell morphology due to thefact dispersed