The synthesis of CNPs had been studied intensively because it has a wide range of interests in industry, biomedical, environment and also food industry. For example, curcumin loaded folate-modified-chitosan-nanoparticles (NPs) have been synthesized and fabricated via self-assembling process 3. The anticancer agent doxorubicin was efficiently encapsulated within the CNP, confirming the feasibility of using this system as a drug delivery vector 4. Wu et al. had prepared Res-loaded CS-TPP particles with the size of 172–217 nm by the ionic crosslinking method Wu, J., Wang, Y.
, Yang, H., Liu, X., & Lu, Z. (2017). Preparation and biological activity studies of resveratrol loaded ionically cross-linked chitosan-TPP nanoparticles. Carbohydrate Polymers, 175, 170–177. https://doi.org/10.
07.058. DOX-loaded CNPs showed excellent anti-proliferative characteristics against HeLa cells with favourable biocompatibility against normal cells Anandhakumar, S., Krishnamoorthy, G.
, Ramkumar, K. M., & Raichur, A. M. (2017). Preparation of collagen peptide functionalized chitosan nanoparticles by ionic gelation method: An effective carrier system for encapsulation and release of doxorubicin for cancer drug delivery. Materials Science and Engineering C, 70, 378–385. https://doi.
org/10.1016/j.msec.2016.09.003. Other than that, chlorogenic acid loaded chitosan nanoparticles with preserved antioxidant activity, controlled release property and enhanced bioavailability 22.
Polyethylene glycol (PEG) modified chitosan (Cs) nanocapsules were developed as a carrier for thymoquinone 26. Safa K.H. et al. reported that warfarin-?-cyclodextrin (WAF-?-CD) loaded chitosan (CS) nanoparticles for transdermal delivery prepared by ionic gelation method tripolyphosphate (TPP) Khalil, S. K. H., El-Feky, G.
S., El-Banna, S. T., & Khalil, W. A. (2012).
Preparation and evaluation of warfarin-?-cyclodextrin loaded chitosan nanoparticles for transdermal delivery. Carbohydrate Polymers, 90(3), 1244–1253. https://doi.org/10.1016/j.carbpol.2012.06.
056. Apart from that, olanzapine loaded chitosan nanoparticles for a nose to brain targeting were prepared by ionic gelation of chitosan with tripolyphosphate anions Ruby, J. J., & Pandey, V. P.
(2016). Formulation and evaluation of olanzapine loaded chitosan nanoparticles for a nose to a brain targeting an in vitro and ex vivo toxicity study. Journal of Applied Pharmaceutical Science, 6(9), 034–040.
S. Moideen et al. prepared atenolol encapsulated chitosan nanoparticles by using ionic gelation technique which involves chitosan with tripolyphosphate anions . Moideen, S. M.
, & Kuppuswamy, S. (2014). World Journal of Pharmaceutical Research SIGNIFICANCE OF NANOPARTICLE DRUG DELIVERY SYSTEM THAN CONVENTIONAL DRUG DELIVERY SYSTEM OF, 3(4), 995–1019.. D. Madan et al. reported ibuprofen encapsulated in chitosan microspheres by using glutaraldehyde as cross-linker via different methods and varying the process conditions 23.
Other than that, the encapsulation of retinol in chitosan nanoparticles minimized the irritation and toxicity of retinol, and the retinol-loaded chitosan nanoparticles can be potentially used for acne and anti-wrinkle treatment . Manuscript, A., & Prospects, F. (2014). NIH Public Access, 11(3), 393–407. https://doi.org/10.1002/wnan.1211.Polymeric